Marianne K. Poxleitner, Ph.D.

Professor of Biology

My primary goal, as a professor, is to share my passion for science with our students. I help them understand what is known and encourage their curiosity of what is unknown. As a scientist, I use classic molecular and cellular biological techniques to...

Picture of Dr. Marianne Poxleitner

Contact Information

  • Spring 2022
    Monday: 10:00-11:00 am
    Tuesday: 10:00 am-12:00 pm
    Thursday: 2:00-3:00 pm

  • (509) 313-5547

Education & Curriculum Vitae

MBA, Gonzaga University School of Business 

Postdoctoral, University of California, Berkeley, Department of Molecular and Cell Biology

Ph.D., Washington State University, Institute of Biological Chemistry, Molecular Plant Sciences

M.S., Colorado State University, Department of Horticulture

B.S., Colorado State University, Department of Horticulture, Graduated Magna cum laude in Scientific Horticulture, Botany 

Courses Taught

B105: Information Flow in Biological Systems

B015L: Phage Discovery Lab

B106: Energy Flow in Biological Systems

B207: Genetics

B351: Advanced Cell Biology

B360: Plant Biology


My primary goal, as a professor, is to share my passion for science with our students. I help them understand what is known and encourage their curiosity of what is unknown. As a scientist, I use classic molecular and cellular biological techniques to investigate various aspects of biology. I currently have two main areas of inquiry in my lab including the study of bacteriophage, viruses that kill bacteria, and genetic modifications of conifer trees to enhance their natural characteristics.

Books:

Poxleitner M, Pope W, Jacobs-Sera D, Sivanathan V, Hatful GF. (2018). Phage Discovery Guide (2nd Edition, eText), Howard Hughes Medical Institute, Chevy Chase, MD. 
Poxleitner M, Pope W, Jacobs-Sera D, Sivanathan V, Hatful GF. (2016). Phage Discovery Guide (1st Edition, print), Howard Hughes Medical Institute, Chevy Chase, MD. 

Peer Reviewed Journal Articles:

Hoag, TP, DeKlotz JP, Steel B, Ettinger WF, Butela KA, Anders KR, Poxleitner M, (2022). Comparison and evaluation of EG cluster Microbacterium foliorum bacteriophage isolated at Gonzaga University. Microbiology Resource Announcements (accepted 3/22).

Fitzgerald S, Johnson E, Storz S, Ballard C, Battaglia S, Boice M, Bramwell-Butcher J, Dedinsky M, DeKlotz J, Diaz I, Engley A, Ernst L, Gonzales E, Groscost A, Grosser P, Haider A, Harrison M, Husler K, Lau JR, Monlux M, Paratore J, Ruesch T, Schlesinger M, Scholes A, Poxleitner M, Anders KR. (2021). Genome sequences of subcluster M2 mycobacteriophages Estes and Aziz. Microbiology. Resource Announcements doi: https://doi.org/10.1128/MRA.00104-21

Dedrick RM, Jacobs-Sera D, Bustamante CA, Garlena RA, Mavrich TN, Pope WH, Reyes JC, Russell DA, Adair T, Alvey R, Bonilla JA, Bricker JS, Brown BR, Byrnes D, Cresawn SG, Davis WB, Dickson LA, Edgington NP, Findley AM, Golebiewska U, Grose JH, Hayes CF, Hughes LE, Hutchison KW, Isern S, Johnson AA, Kenna MA, Klyczek KK, Mageeney CM18, Michael SF16, Molloy SD15, Montgomery MT1, Neitzel J19, Page ST20, Pizzorno MC, Poxleitner MK, Rinehart CA, Robinson CJ, Rubin MR, Teyim JN, Vazquez E, Ware VC, Washington J, Hatfull GF.(2017). Prophage-mediated defense against viral attack and viral counter-defense. Nature Microbiology. 2. 16251. 10.1038/nmicrobiol.2016.251.

David I. Hanauer, Mark J. Graham, SEA-PHAGES, Laura Betancur, Aiyana Bobrownicki, Steven G. Cresawn, Rebecca A. Garlena, Deborah Jacobs-Sera, Nancy Kaufmann, Welkin H. Pope, Daniel A. Russell, William R. Jacobs, Viknesh Sivanathan, David J. Asai, and Graham F. Hatfull. (2017) An Inclusive Research-Education Community (iREC): Impact of the SEA-PHAGES program on research outcomes and student learning. Proc Natl Acad Sci U S A 114: 13531-13536. doi: 10.1073/pnas.1718188115 
(Gonzaga faculty co-authors in the SEA-PHAGES consortium include Poxleitner, Marianne and  Anders, Kirk.) 

Anders, KR, Murphy, AM*, Ettinger, WF, Kempthorne, D*, Kittridge, C*, Kures, A*, Lundgren, S*, Masters, J*, Noyes, R*, Winters, C*, Yazzolino, P*, Ziebert, K*, Haydock, J, Hayes, S, Garlena, RA, Russell, DA, Poxleitner, MK, Ettinger, A-SH. (2017) Genome Sequences of Cluster K Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson. Genome Announcements 5:e01388-16. doi: 10.1128/genomeA.01388-16.  

Staub, NL, Poxleitner, M, Braley, A, Smith, A, Pribbenow, CM, Jaworski, L, Lopatto, D, Anders, KR. (2016) Scaling Up: Adapting a Phage-Hunting Course to Increase Participation of First-Year Students in Research. CBE Life Sciences Education. 15(2). pii: ar13. doi: 10.1187/cbe.15-10-0211.

Steven G. Cresawn, Welkin Pope, Deborah Jacobs-Sera, Charles Bowman, Daniel Russell, Rebekah M. Dedrick, Tamarah Adair, Kirk R. Anders, Sarah Ball, Dave Bollivar, Caroline Breitenberger, Sandra H. Burnett, Kristen Butela, Deanna Byrnes, Kathleen A. Cornely, Luke Daniels, David Dunbar, Ann M. Findley, Chris R. Gissendanner, Urszula P. Golebiewska, Grant A. Hartzog, J. J. Robert Hatherill, Lee E. Hughes, Chernoh S. Jalloh, Sphindile L Khambule, Rodney King, Christina King Smith, Karen Klyczek, Greg P. Krukonis, Christian Laing, Jonathan S Lapin, A. Javier Lopez, Sipho M. Mkhwanazi, Sally Molloy, Vanisha Munsamy, Eddie Pacey, Kimenthrie Pillay, Ruth Plymale, Marianne Poxleitner, Nathan Reyna, Joel Schildbach, Joseph Stukey, Sarah B. Taylor, Vassie C. Ware, Amanda L Wellmann, Daniel Westholm, Thabiso S. Zikalala, Roger W. Hendrix, Graham F. Hatfull. (2015) Comparative Genomics of Cluster O mycobacteriophages. PLoS ONE 10(3): e0118725. doi: 10.1371/journal.pone.0118725

Welkin Pope, Charles Bowman, SEA-PHAGES1, PHIRE, K-RITH MGC, Deborah Jacobs-Sera, Daniel A. Russell, Steven Cresawn, William R. Jacobs Jr., Jeffrey G. Lawrence, Roger W. Hendrix, and Graham F. Hatfull. (2015) Dramatic variation in phage genome structures revealed by whole genome comparisons. eLIFE 4:e06416
(Gonzaga faculty and student authors in the SEA-PHAGES program include: Marianne Poxleitner, Kirk Anders, Ann-Scott Ettinger, Joseph Haydock, Shawna Pfeif*, Nicholas Contreras*, Riley Benoit*, Samantha Blake*, Cayla Boisseranc*, Erinleigh Caughron*, John, Culver*, Samuel Gordon*, Francis Green*, Alexis Holder*, Kevin Johnston*, Staci Koberstein*, Suzanne, Lacour*, Mary McAleer*, Taylor Oswald*, Joseph Ryan*, Monica Schroll*, Chelsea Stone*, Graciela Tobar*, Tara Togstad*, Mackenzie Bredereck*, Emily Carlson*, John Chase*, Ashley Connors*, Zachary Damby*, Marshall Davis*, Patrick Hashiguchi*, John Henderson*, Erin Lapsansky*, Dave Lee*, Alyssa Logan*, Mariah Minder*, Olivia, Schuele*, Elizabeth Toscan*, William Wilde*, Sarah Yeend*, Megan Batty*, Janella Bermudez*, Savannah Bukant*, Katherine Carr*, Ekaterina Dasiuk*, Kayci Fudge*, Jacob Gries*, Nathaniel Guilford*, Aspen Hirsch*, Chandler Jensen-Cody*, Jackson Jones*, Taylor Jordan*, Aleksey Kozlov*, Nina Montoya*, Alexandra Murphy*, Petros Raygoza*, Laura Shellooe*, Helen Xun*)

Hatfull, G.F., Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program*, KwaZulu-Natal Research Institute for Tuberculosis and HIV (K-RITH) Mycobacterial Genetics Course, University of California Los Angeles  Research Immersion Laboratory in Virology, Phage Hunters Integrating Research and Education (PHIRE) program. (2013). The complete genome sequences of 63 mycobacteriophages. Genome Announc 1, 10.1128/genomeA.00847-13
(Gonzaga faculty and student authors in the SEA-PHAGES program include: Bredereck,M.L.*, Carlson,E.A.*, Chase,J.B.*, Connors,A.*, Damby,Z.S.*, Davis,M.J.*, Hashiguchi,P.K.*, Henderson,J.H.*, Lapsansky,E.R.*, Lee,D.K.*, Logan,A.M.*, Minder,M.A.*, Schuele,O.K.*, Toscan,E.D.*, Wilde,W.T.*, Yeend,S.E.*, Poxleitner,M.K., Anders,K. R.)

Hatfull, G. F., Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program*, KwaZulu-Natal Research Institute for Tuberculosis and HIV (K-RITH) Mycobacterial Genetics Course, Phage Hunters Integrating Research and Education (PHIRE) program. Complete genome sequences of 138 mycobacteriophages. (2012). J Virol 86:2382-2384.
(Gonzaga faculty and student authors in the SEA-PHAGES program include: Anders, K. R., Poxleitner, M.K., Benoit R.P.*, Blake S.A.*, Boisseranc C.J.*, Caughron E.K.*, Culver J.C.*, Gordon S.*, Green F.G.*, Holder A.J.*, Johnston K.R.*, Koberstein S.L.*, Lacour S.G.*, McAleer M.C.*, Oswald T.*, Ryan J.S.*, Schroll M.*, Stone C.A.*, Tobar G.*, Togstad T.L.*, Pfeif S.M.*, Contreras N.*)

Marianne K. Poxleitner, Meredith L. Carpenter, Joel J. Mancuso, Chung-Ju R. Wang, Scott Dawson, and W. Zacheus Cande. Evidence for Karyogamy and Exchange of Genetic Material in the Binucleate Intestinal Parasite Giardia intestinalis. (2008). Science.  319:1530-1533.

Marianne Poxleitner, Scott Dawson, and W. Zacheus Cande, Cell Cycle Synchrony 
in Giardia lamblia. (2008). Eukaryotic Cell 7(4):569-574.

Marianne Poxleitner, A. Lacey Samuels, John Browse, and John C. Rogers, A Role for Caleosin in Oil Body-Vacuole Interactions During Seed Germination, (2006). Plant Journal. 47: 917-933.

Mohammed Oufattole, Joon Ho Park, Marianne Poxleitner, Liwen Jiang, and John C. 
Rogers, Selective Membrane Protein Internalization Accompanies Movement from the Endoplasmic Reticulum to the Protein Storage Vacuole Pathway in Arabidopsis. (2005). Plant Cell. 17: 3066-3080.

Marianne Poxleitner. Effect of Irrigation Deficit and Ethephon Application on Color Enhancement of Vitis Vinifera. (1999).  Booklet published for the Colorado Grape Growers Association

Annotated Genomes Published in GenBank (* indicates undergraduate author):

Deklotz,J.P*., Hoag,T.P*., Steele,B.K*., Poxleitner,M.K., Butela,K.A., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. (2021) Complete genome sequence of Microbacterium phage Teehee. Genbank Accession MW712727.1.
 
Deklotz,J.P*., Hoag,T.P*., Steele,B.K*., Poxleitner,M.K., Butela,K.A., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. (2021) Complete genome sequence of Microbacterium phage Quammi. GenBank Accession MW712728.1.

Deklotz,J.P*., Hoag,T.P*., Steele,B.K*., Poxleitner,M.K., Butela,K.A., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. (2021) Complete genome sequence of Microbacterium phage StrawberryJamm. GenBank Accession MW712724.1.

Ruesch,T*., Stepniewski,C*., Ballard,C*., Battaglia,S*., Diaz,I., Engley,A*., Erickson,A*., Ernst,L*., Gonzales,E*., Haider,A*., Harrison,M*., Moore,J*., Paratore,J*., Rafanan,A*., Storz,S*., Poxleitner,M.K., Anders,K.R., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. (2020) Complete genome sequence of Mycobacterium phage Aziz. GenBank Accession MT658802.1.

Jones,J*., Bates,R*., Cardinale,D*., Cook,D*., Gunelson,A*., Harder,B*., Jimenez,S*., Lindsey,T*., Lubisich,J*., Mudge,J*., Pendilla,L*., Ramos,M*., Reisch,L*., Steinhilber,E*., Urbanek,K*., Zaragoza,V*., Poxleitner,M.K., Ettinger,A.-S.H., Ettinger,W.F., Schaff,J.E., Dashiell,C.L., Macialek,J.A*., Anders,K.R., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2020) Complete genome sequence of Mycobacterium phage Coco12. Genebank Accession NC051644.1

Allen,T*., Groscost,A*., Boice,M*., Bramwell-Butcher,J*., Davis-Nicholson,M*., Dedinsky,M*., DeKlotz,J*., Gardner,J*., Grosser,P*., Husler,K*., Lau,J.R*., Monlux,M*., Schlesinger,M.K*., Scholes,A*., Waughman,L*., Poxleitner,M.K., Anders,K.R., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F.
(2020) Complete genome sequence of Mycobacterium phage Estes. GenBank Accession MT657341.1.

Carlson,E*., Bamesberger,N*., Battaglia,Z*., Budnack,T*., Cardinale,D*., Chung,C*., Diesslin,M.T*., Fenner,I*., Goligoski,M*., Lumsden,M*., Murray,C*., Schwander,K*., Poxleitner,M.K., Ettinger,A.-S.H., Ettinger,W.F., Anders,K.R., Schaff,J.E., Dashiell,C.L., Macialek,J.A., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2020) Complete genome sequence of Mycobacterium phage Gandalph. Genbank Accession: NC051662.1

Murphy,A*., Cottrell,A*., Eagle,H*., Glenn,A*., Marsh,C*., Poisson,J*., Schott,J*., Steele,B*., Sunitsch,G*., Tsybulnik,D*., Uchytil,C*., Vue,L*., Poxleitner,M., Ettinger,A.-S.H., Anders,K.R., Schaff,J.E., Dashiell,C.L., Macialek,J.A., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2020) Complete genome sequence of Mycobacterium phage Phanphagia, GenBank Accession MT114167.

Shellooe,L*., Bartley,R*., Buttafoco,A*., Evard,R*., Fellin,M*., Poisson,J*., Schneller,A*., Poxleitner,M., Ettinger,W.F., Ettinger,A.-S.H., Anders,K.R., Schaff,J.E., Dashiell,C.L., Macialek,J.A., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2020) Complete genome sequence of Mycobacterium phage Veteran. GenBank Accession MT114164.1

Anderson,C*., Anderson,P*., Beaty,B*., Beekly,B*., Bell,A*., Bell,D*., Bernicchi,A*., Blomquist,M. *, Briggs,H*., Carlson,A*., Clark,A*., Constien,J*., Dauenhauer,C*., Delatorre,V*., Ellis,I*., Goins,J*., Goodman,B*., Heckman,M*., Henley,B*., Hensle,E*., Hoitink,M*., Kern,M*., Kratochvil,T*., Lapsansky,A*., Lucassen,A*., Maccarrone,A*., Mathrani,O*., McNulty,K*., Meister,R*., Miess,O*., Mills,S*., Nakamine,K*., Nemri,R*., Nintzel,R*., Novajovsky,A*., Ong,G*., Owens,S*., Pennington,K*., Permito,R*., Pihl,B*., Raygoza,P*., Rhoten,S*., Riddelle,K*., Rutledge,C*., Ryan,N*., Schneider,C*., Schulz,M*., Snider,J*., Soucek,K*., Stoddard,T*., Thomas,K*., Tiatragul,S*., Tomisato,S*., Uhder,S*., White,D*., Wilde,L*., Wilson,E*., Wimbush,I*.,  Dardis,C*., Murphy,A.M*., Virk,M*., Young,E*., Ettinger,A.-S.H., Ettinger,W.F., Poxleitner,M.K., Anders,K.R., Serrano,M.G., Buck,G., Lee,V., Wang,Y*., Carvalho,R*., Voegtly,L*., Shi,R*., Duckworth,R*. Johnson,A*., Loviza,R*., Walstead,R*., Shah,Z*., Kiflezghi,M*., Wade,K*., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H.,  Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2015) Complete genome sequence of Mycobacterium phage Phreak. GenBank Accession KT347314.2.

Biederman,W., Baliraine,F.N., Batchenkova,N., Beeser,A., Bonetta,L., Caslake,L., Ceballos,R.M., Chung,H.-M., Conant,S.B., Dalia,R., D'Angelo,W., DeCourcy,K., Eckardt,M., Fogarty,M.P., Frederick,G.D., Gainey,M.D., Hendrickson,H., Herren,C.D., Ho,E., Jenson,M., Kagey,J.D., Leadon,S., Little,J.L., Joshua,M., Mavrodi,D.V., Moberg-Parker,J., Olapade,O., Overath,R.D., Pape-Zambito,D., Pirino,G., Ryder,E.F., Saha,S., Saville,K., Schmeier,S., SmithCaldas,M., Sunnen,C.N., Thomson,J., Vargas-Blanco,D.A., Voshell,S.M., Wallen,J.R., Whitefleet-Smith,J.L., Anders,K.R., Edgington,N.P., Asai,D.J., Bradley,K.W., Poxleitner,M., Sivanathan,V., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Cresawn,S.G., Hendrix,R.W. and Hatfull,G.F. (2015) Complete genome sequence of Mycobacterium phage NaSiaTalie GenBank Accession KU297783.1.

Batty,M*., Bermudez,J*., Bukant,S.K*., Carr,K.E*., Dasiuk,E.A*., Fudge,K.A*., Gries,J*., Guilford,N.C*., Hirsch,A.L*., Jensen-Cody,C.W*.,Jones,J.E*., Jordan,T.K*., Kozlov,A.V*., Montoya,N.R., Murphy,A.M*.,Raygoza,P.M*., Shellooe,L.T*., Xun,H*., Poxleitner,M.K., Anders,K.R.,Serrano,M.G., Buck,G., Lee,V., Wang,Y., Carvalho,R., Voegtly,L.,Shi,R., Duckworth,R., Johnson,A., Loviza,R., Walstead,R., Shah,Z.,Kiflezghi,M., Wade,K., Braun,M.A., Delesalle,V.A., Hughes,L.E.,Ware,V.C., Bradley,K.W., Barker,L.P., Asai,D.J., Bowman,C.A.,Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2014) Mycobacterium phage Phoxy, complete genome. GenBank Accession KP017310.

Batty,M*., Bermudez,J*., Birge,J*., Bukant,S.K*., Carr,K.E*.,Coghlan,K*., Dasiuk,E.A*., Freeman,C*., Freitas,V*., Fudge,K.A*.,Gries,J*., Grome,K*., Guilford,N.C*., Hirsch,A.L*., Ibele,A*., Jensen-Cody,C.W*., Jones,M*., Jones,J.E*., Jordan,T.K*., Kangas,L*., Karam,A*., Kozlov,A.V*., LeTendre,N*., Massie,A*., Matern,J*., Moe,R*., Montoya,N.R*., Moran,M*., Murphy,A.M*., Neelay,O*., Obrinsky,T*., Parker,J., Price,K., Raygoza,P.M., Reese,B., Shafer,A., Shellooe,L.T., Uppendahl,K., Van Deynze,B., Wiest,K*., Xun,H*., Young,C*., Ettinger,A.-S.H., Haydock,J., Poxleitner,M.K.,Anders,K.R., Serrano,M.G., Buck,G., Lee,V., Wang,Y., Carvalho,R., Voegtly,L., Shi,R., Duckworth,R., Johnson,A., Loviza,R.,Walstead,R., Shah,Z., Kiflezghi,M., Wade,K., Braun,M.A.,Delesalle,V.A., Hughes,L.E., Ware,V.C., Bradley,K.W., Barker,L.P.,Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D.,Hendrix,R.W. and Hatfull,G.F. (2014) Complete Genome sequence of Mycobacterium phage Spike509. GenBank Accession KP017311.

Bredereck,M.L.*, Carlson,E.A.*, Chase,J.B.*, Connors,A.*, Damby,Z.S.*, Davis,M.J.*, Hashiguchi,P.K.*, Henderson,J.H.*, Lapsansky,E.R.*, Lee,D.K.*, Logan,A.M.*, Minder,M.A.*, Schuele,O.K.*, Toscan,E.D.*, Wilde,W.T.*, Yeend,S.E.*, Poxleitner,M.K., Anders,K.R., Bradley,K.W., Khaja,R., Lewis,M.F., Barker,L.P., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2013) Complete genome sequence Mycobacterium phage SiSi. GenBank KC661278.1

Fried-Petersen,H., Adair,T.L., Anders,K.R., Aley,S.B., Bratsch,S.A., Clase,K.L., Coleman,J.M., Debro,L.H., Dellis,S., Fang,Y., Findeis,S., Gibbon,B.C., Golebiewska,U.P., Grillo,W.H., Grose,J.H., Hester,A., Hollowell,G.P., Kearney,S., Kelly,J., Klyczek,K., Kuleck,G., Londono,J.A., Mogen,K., Monti,D.L., Murdock,C., Ovalle,R., Pfeif,S., Pizzorno,M.C., Poxleitner,M., Reyes,D., Rickus,J.L., Rosas-Acosta,G., Schneider,P., Stowe-Evans,E., Stukey,J., Taylor,M.A., Tollis,M., Wong,C.K., Wu,H., Zimmerman,A.M., Cresawn,S.G., Lee,E., Shaffer,C.D., Barker,L.P., Bradley,K.W., Khaja,R., Lewis,M.F., Jordan,T.C., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2012). Complete genome sequence of Mycobacterium phage Wee, Genbank Accession JN398368.1.

Benoit,R.P.*, Blake,S.A.*, Boisseranc,C.J.*, Caughron,E.K.*, Culver,J.C.*, Gordon,S.*, Green,F.G.*, Holder,A.J.*, Johnston,K.R.*, Koberstein,S.L.*, Lacour,S.G.*, McAleer,M.C.*, Oswald,T.*, Ryan,J.S.*, Schroll,M.*, Stone,C.A.*, Tobar,G.*, Togstad,T.L.*, Pfeif,S.M.*, Contreras,N.*, Anders,K.R., Poxleitner,M.K., Lee,V., Hendricks,S.L., Voegtly,L.J., Wang,Y., Glascock,A.L., Anderson,J., Williamson,S.M., Walstead,R.N., Carvalho,M.R.C., Johnson,A., Buck,G.A., Bradley,K.W., Khaja,R., Lewis,M.F., Barker,L.P., Jordan,T.C., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. (2011) Complete genome sequence Mycobacterium phage GUmbie,. GenBank JN398368.1. 
     

 

My lab investigates bacteriophages, viruses that infect bacteria. Student researchers apply skills learned in B105: Phage Discovery Lab and B207: Genetics Lab to answer advanced phage biology questions. One project investigates mechanisms of bacterial resistance to viral infection. Over the past several billion years the phage-bacteria relationship has been under constant selective pressure for bacteria to evolve ways to escape viral infection and death, and for phages to overcome these mechanisms. Bacteria already infected by a virus are often times resistant, or insensitive, to infection by other phages through a phenomenon known as superinfection immunity. We are using CRISPR/Cas9 to knockout phage genes of interest to identify mechanisms of bacterial insensitivity caused by previous phage infections. A second line of investigation isolates bacteriophages from new bacterial hosts in the phylum Actinobacteria. Students choose a bacterial host, learn to culture the bacteria, then isolate, purify, and genetically characterize a novel phage.