Kylie Allen, Ph.D.

Assistant Professor, Chemistry and Biochemistry

I am a biochemist with specific interests in the unique enzymes and coenzymes involved in methanogenesis.

Profile photo of Assistant Professor Kylie Allen

Contact Information

  • Hughes Hall 230A
  • Fall 2017
    Monday:  4:10-5:10 p.m.
    Tuesday:  12:00-2:00 p.m.
    Wednesday:  4:10-5:00 p.m.

  • 509-313-5839

Education & Curriculum Vitae

Postdoctoral, Biochemistry, Virginia Tech, 2015.

Ph.D., Biochemistry, Washington State University, 2013

B.S., Biology, Eastern Washington University, 2017

Curriculum Vitae

Courses Taught

CHEM 101 General Chemistry

CHEM 101L General Chemistry Laboratory

CHEM 240 Bioanalytical Chemistry

CHEM 240L Bioanalytical Chemistry Laboratory

CHEM 440 Biochemistry


Allen, K. and White, R. (2016) Occurrence and biosynthesis of 3-mercaptopropionic acid in Methanocaldococcus jannaschii. FEMS Microbiology letters 363, fnw217.

Allen, K., Miller, D., Rauch, B., Perona, J., and White, R. (2015) Homocysteine is biosynthesized from aspartate semialdehyde and hydrogen sulfide in methanogenic archaea, Biochemistry 54, 3129-3132.

Feirer, N., Xu, J., Allen, K., Koestler, B., Waters, C., White, R., and Fuqua, C. (2015) A Pterin-Dependent Signaling Pathway Regulates a Dual Function Diguanylate Cyclase-Phosphodiesterase Protein Controlling Surface Attachment in Agrobacterium tumefaciens, mBio, 6(4).

Hu, K., Werner, W., Allen, K., Wang, S. (2015) Single Quantum and Multiple Quantum HCP Nuclear Magnetic Resonance Experiments Demonstrate C-P Bond Formation by PhpK from Kitasatospora phosalacinea, Magnetic Resonance in Chemistry 53, 267-272.

Allen, K., and White, R. (2014) Identification of structurally diverse methanofuran coenzymes in Methanococcales that are both N-formylated and N-acetylated, Biochemistry 53, 6199–6210.

Allen, K., and Wang, S., (2014) Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217, Biochimica et Biophysica Acta. 1844, 2135-2144.

Allen, K., Wegner, G., and White, R. (2014) Discovery of multiple modified F430 coenzymes in methanogens and anaerobic methylotrophic archaea suggests new roles for F430 in Nature. Applied and Environmental Microbiology 80, 6403-6412. (Spotlight Article)

Allen, K., Xu, H., and White, R. (2014) Identification of a unique radical SAM methyltransferase likely involved in methanopterin biosynthesis in Methanocaldococcus jannaschii, Journal of Bacteriology 196, 3315-3323. (Selected by Faculty of 1000)

 Allen, K., and Wang, S. (2014) Initial Characterization of Fom3 from Streptomyces wedmorensis: The Methyltransferase in Fosfomycin Biosynthesis, Archives of Biochemistry and Biophysics 543, 67-73.

Werner, W*.,Allen, K*., Hu, K., Helms, G., Chen, B., and Wang, S. (2011) In Vitro Phosphinate methylation by PhpK from Kitasatospora phosalacinea, Biochemistry 50, 8986-8988.

*Contributed equally

Investigating the function(s) of a modified coenzyme F430 in methanogenic archaea

Coenzyme F430 is required for the final step of methane formation in methanogenic archaea and the first step of methane oxidation in anaerobic methanotrophic archaea. Since methane is both a potent greenhouse gas and a valuable energy source, it is imperative to understand its production and consumption in nature. Recently, a unique variant of coenzyme F430, called F430-3, was discovered in methanogens, which is hypothesized to be involved in reversing methanogensis in these organisms. My research seeks to investigate the potential function(s) of F430-3 and to determine the gene/enzyme involved in the presumed first step of F430-3 biosynthesis. A more complete and deeper understanding of methane metabolism could allow for alternatives to fossil fuels and potential remedies for climate change.